Joshua Knackert

Degree Program: Cellular and Molecular Pathology
Faculty Advisor: Su-Chun Zhang
Phone: (608) 263-9674

Current Research

I am utilizing iPS lines derived from patients with ALS to establish in vitro disease modeling. These lines are being differentiated into the cell of interest in ALS, spinal motor neurons. I am then evaluating the disease and control motor neurons for ALS associated phenotypes. My thesis research plans to utilize two pairs of known mutant ALS iPS lines, SOD1 and TDP-43, along with their isogenic controls to compare how phenotypes differ between the two mutations. This will be accomplished via molecular methods like qPCR, Western blots, and ICC. I also plan to tag proteins of interest with the hopes of watching phenotype progression in real time, and the possibility of incorporation into a drug screening assay. Data gathered from these experiments will likely hint at potential mechanisms involved in the pathology of ALS.


Proc Natl Acad Sci U S A. 2013 Jun 11;110(24):9962-7. doi: 10.1073/pnas.1216575110. Epub 2013 May 28. Deficits in human trisomy 21 iPSCs and neurons. Weick JP1, Held DL, Bonadurer GF 3rd, Doers ME, Liu Y, Maguire C, Clark A, Knackert JA, Molinarolo K, Musser M, Yao L, Yin Y, Lu J, Zhang X, Zhang SC,Bhattacharyya A.

Abstract (PDF)

Resume (PDF)