Tucker J. Carrocci 

Tucker J. Carrocci

Degree Program: Biochemistry
Faculty Supervisor: Aaron A. Hoskins
Phone: (608) 262-3040
Email: carrocci@wisc.edu

 

 

Current Research

In vivo fluorescent labeling has proven useful in gaining a molecular understanding of many biological systems. Extension of this technique to RNA systems has been plagued by the lack of fluorescent reporter motifs. A previously developed method using fluorescent proteins to study messenger RNA (mRNA) utilizes RNA binding sites of the MS2 bacteriophage coat protein cloned into an untranslated region of the target RNA molecule and the coexpression of green fluorescent protein (GFP) fused MS2-­-binding protein. Despite the successful application of this method to a number of biological questions, it is not without drawbacks such as background fluorescence from unbound MS2-­-GFP, the large size of the reporter system, and weak fluorescence of GFP compared to organic fluorophores that emit light in the visible and near-­-IR spectrums. Alternative methods would prove useful for studying systems not amenable to visualization by MS2-­-GFP. We are in the process of creating tools in which RNA can be visualized by small molecule or protein fluorescence and either by direct excitation or fluorescence resonance energy transfer (FRET).

Abstract (PDF)

Resume (PDF)

 

 

 


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