Current Research

Defining the RNA-binding activities of the translational Repressor Bicaudal-C

Bicaudal-C (Bic-C) is an important RNA binding protein required for embryonic development and the development and function of specific adult organs in species ranging from flies to humans. Previous work from our lab established that Xenopus Bic-C binds to a 32-nucleotide region within the 3’UTR of the maternal mRNA encoding the Cripto-1 protein and represses its translation. I am defining the both the protein features of Bicaudal-C involved in binding as well as the important sequence features of the Bic-C binding site at nucleotide resolution using a mutational approach in conjunction with electrophoretic mobility shift and fluorescence anisotropy assays to measure Bic-C binding. I have so far defined the importance of the KH domains of Bicaudal-C involved in binding RNAs.

Selected Publications

Sheets MD, Fox CA, Dowdle ME, Blaser SI, Chung A, Park S. Controlling the Messenger: Regulated translation of maternal mRNAs in Xenopus laevis development. Adv Exp Med Biol. 2017;953:49-82. PMID: 27975270.

Dowdle ME, Imboden SB, Park S, Ryder SP, Sheets MD. Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes. J Vis Exp. 2017 Jul 28;(125). PMID: 28784977.