Clara Frazier

Current Research

Developing an enzymatic tool for identifying protein C-termini

Post-translational modifications such as proteolysis occur are spatially and temporally modified within living cells. Analysis of protein C-termini can provides valuable information about the regulation of biological processes, especially proteolysis. Identifying C-termini is especially challenging due to the less reactive nature of the carboxy terminus and lack of applicable tools. My research is focused on engineering an enzyme tool to recognize and tag protein C-termini within living cells. I am currently working with members of the MccB enzyme family which catalyze adenylation at the C-terminus of their substrate peptides.