Development and Application of Improved Strategies for Proteomic Profiling
Mass spectrometry-based profiling is an ideal method for broad profiling the proteinaceous makeup of any biological sample. My first research project was the improved analysis of human blood plasma. The methods that I developed were used to analyze a longitudinal sample set comprised of healthy and cognitively impaired individuals for the purpose of profiling the onset of Alzheimer’s Disease. These data were presented at a national conference in late January of 2017 and will fit into a larger manuscript that will include metabolomics, lipidomics, and neural imaging of the same patient set. We can also apply proteomic methodologies to broadly look at protein modifications (i.e. phosphorylation, acetylation, ubiquitination, glycosylation). I have had a number of successful projects profiling the phosphoproteome for the characterization of previously unstudied phosphatases, as well as, to study the signaling events that occur in response to muscle immobilization and, subsequently, atrophy. In parallel, I am working on our lab’s methods for studying protein glycosylation, including improved enrichment techniques and peptide/glycan fragmentation methods for better sequence identification.