Position title: Integrated Program in Biochemistry
- James Keck
Elucidating the regulatory steps in the PriA-PriB-DnaT Replication Restart Pathway in E. coli
Restarting failed DNA replication is crucial for maintaining genome integrity in E. coli. The PriA-PriB-DnaT replication restart pathway identifies and remodels abandoned replication forks to efficiently recruit and reload the replicative helicase, DnaB, at sites far from the origin of replication (oriC). The precise regulatory steps in how DNA replication is restarted remains to be known. Using single-molecule Forster resonance energy transfer (smFRET) and cyro-electron microscopy (cryoEM), conformational states and thermodynamic parameters will reveal how DNA replication is restarted selectively at abandoned DNA replication structures, further expanding our knowledge on genome maintenance processes.